Table 2.

Kinetic parameters of PSA peptide substrates

Group	PSA substratea	Km (µm)	kcat (s−1)	kcat:Km (s−1 m−1)
1	KGISSQY	160	0.043	270
	SRKSQQY	90	0.023	260
2	EHSSKLQ	1165	0.012	10.6
	HSSKLQ	470	0.011	23.6
	SKLQ	813	0.020	24.6
	ATKSKQH	1310	0.0091	6.9
3	KGLSSQC	300	0.0017	5.6
	LGGSSQL	900	0.0037	4.1

^aPSA-mediated hydrolysis of substrates in buffer [50 mm Tris and 0.1 m NaCI (pH 7.8)].