Figure 1.

High molecular weight (>95 kDa) antigens predominantly recognized by chagasic patients antibodies. SDS-PAGE was performed in 12% polyacrilamide gel and then transferred into a nitrocellulose membrane for Western blot analysis of chagasic and leishmaniasic patients' sera (dilution 1 : 500) with sonicated total extract of epimastigotes of T. cruzi and peroxide-conjugated anti-human IgG (1 : 10000). Predominant recognition of antigens with molecular weights >95 kDa by chagasic patients' antibodies and less recognition by leishmaniasic patients' antibodies of the same antigens is highlighted (black box).