Figure 4.

Western blot analysis of chagasic and leishmaniasic patients' sera using different extracts as antigens. (a) SDS-PAGE of sonicated extract was performed in 12% polyacrylamide gel and then transferred into a nitrocellulose membrane for Western blot analysis of the serum samples (dilution 1 : 500) and peroxide-conjugated anti-human IgG (1 : 10000). 100% of the leishmaniasic samples exhibit cross-reaction. (b) SDS-PAGE of sonicated, Triton X-100, NP40, and urea-thiourea extracts was performed in 6% polyacrilamide gel and then transferred into a nitrocellulose membrane for Western blot analysis of the serum samples (1 : 750) and the peroxide-conjugated anti-human IgG (1 : 10000). Unspecific proteins recognized by the negative control were not considered for the final analysis.