Figure 5.

Western blot analysis of chagasic and leishmaniasic patients' sera using deglycosylated extracts. Enzymatic deglycosylation was performed with (A, C) O-glycosidase and α(2 → 3,6, 8,9)-neuraminidase; (B, D) β(1 → 4)-galactosidase and β-N-acetylglucosaminidase. 1 and 2 correspond to chagasic patients' sera samples and 3 and 4 correspond to leishmaniasic patients' sera samples (dilution 1 : 750) and peroxide-conjugated anti-human IgG (1 : 10000). Lectin PT was used as a positive control for carbohydrate recognition. A positive serum sample was also used as a positive control for antigenic recognition.