Table 2.
Glycoproteins present in high molecular weight extracts fraction.
| Extraction agent | Lectin | Recognized carbohydrate | Glycoprotein M.W. (kDa) recognized also by chagasic patients' antibodies |
|---|---|---|---|
| Sonication | Con A | αMan > αGlc > GlcNAc | >250, 190, 160, 130, 120, 110, and 100 |
| WGA | GlcNAc, NeuNAc | >250, 190 | |
| Jacalin | αGal→Ome | >250, 190, 130, 120, 110, and 100 | |
| PT | GalNAc, βGal | — | |
| PNA | βGal (1→3) galNAc | — | |
|
| |||
| Triton X-100 | Con A | αMan > αGlc > GlcNAc | >250, 170, 160, 140, 120, and 110 |
| WGA | GlcNAc, NeuNAc | >250 | |
| Jacalin | αGal→Ome | — | |
| PT | GalNAc, βGal | — | |
| PNA | βGal (1→3) galNAc | — | |
|
| |||
| NP40 | Con A | αMan > αGlc > GlcNAc | >250, 170, 150, 120, 115, and 100 |
| WGA | GlcNAc, NeuNAc | — | |
| Jacalin | αGal→Ome | — | |
| PT | GalNAc, βGal | — | |
| PNA | βGal (1→3) galNAc | — | |
|
| |||
| Urea-thiourea | Con A | αMan > αGlc > GlcNAc | >250, 100 |
| WGA | GlcNAc, NeuNAc | — | |
| Jacalin | αGal→Ome | 130, 100 | |
| PT | GalNAc, βGal | — | |
| PNA | βGal (1→3) galNAc | — | |
Lectin blot assays were performed to determine carbohydrate content in each fraction with a 5 μg/mL concentration of lectins Con A, WGA, Jacalin, PT, PNA, and MAA and the peroxidase-conjugated streptavidin (dilution 1 : 2000).