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. 2014 Aug 3;11:137. doi: 10.1186/1743-422X-11-137

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Copyright © 2014 Aebischer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analytical sensitivity and linear dynamic range of manual and rapid automated extraction methods. Dilution series of serum samples positive for SBV-RNA (A) or BVDV-RNA (B) as well as dilution series of EDTA-blood samples positive for SBV-RNA (C) or BVDV-RNA (D) were used to evaluate the extraction efficiency of the rapid extraction protocols in comparison to the standard manual extraction protocol. Each dilution step was extracted in duplicate and the extracted RNA from each panel was tested in the same RT-qPCR run. R² values as defined by regression analysis are indicated for each method.