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PCR efficiency after manual and automated extraction
| Target | Material |
% PCR efficiency |
|||
|---|---|---|---|---|---|
| Manual | EZ1 | BS 15 | KF Duo | ||
| SBV |
Blood |
99.6 |
97.0 |
86.1 |
101.2 |
| Serum |
97.8 |
121.5 |
87.1 |
112.7 |
|
| BVDV | Blood |
112.4 |
99.3 |
88.8 |
84.2 |
| Serum | 95.0 | 98.2 | 92.8 | 110.8 | |
10-fold dilution series of SBV- and BVDV-positive blood and serum samples which were extracted in duplicate; PCR efficiencies of the respective RNA extracts were calculated from the slope of the standard curves.
