FIGURE 1.
Nerve growth factor colocalizes extensively with inhibitory cell markers. Immunoreactive cells in the (A) primary motor cortex and (B) prefrontal cortex. Images show that NGF-labeled cells (green) colocalize extensively with GAD 65/67-labeled cells (red). White arrows show an example cell immunoreactive for both NGF and GAD 65/67 in each cortical region. Blue arrows show cells immunoreactive for GAD 65/67 only. (C) Magnified image of area inside white box in (B). (D) X–Y–Z reconstruction of the double-labeled cell indicated in (C). Green and Blue lines indicate dissection levels in XZ and YZ planes, respectively. Scale bars = 25 μm.