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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Integr Biol (Camb). 2010 Jul 22;2(0):346–353. doi: 10.1039/c0ib00016g

Fig. 4.

Fig. 4

Dependence of stem cell differentiation frequency maps on pattern geometry. (A) ALP activity of human adipose-derived stem cells (hASCs) in osteogenic differentiation medium for 3 days. Frequency maps (1–4) of ALP activity are shown for stem cells on rectangles measuring 1000 × 200 μm, 1000 × 100 μm, 500 × 200 μm, and 500 × 100 μm; the corresponding average ALP activity along the length of each pattern is shown in 5–8. (B) ALP activity of hASCs on rings. Stem cell ALP activities on ring patterns with different sizes are shown in the frequency maps (from left to right 1–4: inner diameter D=1000 μm, width W=200 μm; D=1000 μm, W=100 μm; D=500 μm, W=200 μm; and D=500 μm, W = 100 μm), and the corresponding ALP activity profile across the radius is shown in 5–8. Double-ended arrows indicate the patterned cell-adhesive regions. (# significantly different from the peak ALP activity near two ends in the same rectangle, * significantly from other groups, and ** significantly different from the ring with an inner diameter of 500 μm and width of 200 μm; p < 0.05).