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. 2014 Jul 17;2014:216094. doi: 10.1155/2014/216094

Figure 2.

Figure 2

(a, b) SMN immunoreactivity in fibroblasts obtained from the proband (scale bar = 10 μm): a faint (red) staining was present within nuclear structures (actin filaments counterstained in green) in vehicle-treated cells (VEH), while VPA 5 mM/72 h induced an apparent density increase of SMN-positive nuclear aggregates; (c) acetyl-histone H3 association with SMN promoter was increased in fibroblast obtained from the proband following exposure to VPA 5 mM/72 h, as semiquantified by ChIP assay (*P < 0.05); (d) SMN-like immunoreactive content in fibroblasts obtained from the proband is increased following in vitro exposure to VPA, as semiquantified by Western blotting (°P < 0.05); (e) (Western blotting) SMN-like immunoreactive content in fibroblasts obtained from 12 SALS patients is reduced with respect to 9 matched controls (*P = 0.01), as also shown by (f) representative immunoreactive signals (10 μg of total protein) obtained in 5 different SALS patients and 4 CTRL subjects.