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. 2014 Aug 7;8:214. doi: 10.3389/fncel.2014.00214

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Copyright © 2014 Sutinen, Korolainen, Häyrinen, Alafuzoff, Petratos, Salminen, Soininen, Pirttilä and Ojala.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An example sets of 2D-DIGE protein profiles of SH-SY5Y cells. (A) Differentiated SH-SY5Y cells, at the 48 h time-point where the number of protein spots was the greatest. The arrow heads indicate the picked proteins, done from the silver stained gels; protein ID: (1) PRX2, (2) PRX3, (3) PRX6, (4) DJ-1, (5) BLVRA, (6) DDAH1, (7) DDAH2, (8) TIMP2, (9) SEPT2, (10) ENOA, (11) ENOG, (12) 14-3-3γ, (13) 14-3-3ε, (14) RUFY3, (15) CRMP2. (B) Undifferentiated SH-SY5Y neuroblastoma cells showed the least number of spots, in comparison to differentiated SH-SY5Y cells in (A). (C) Protein profile of the culture medium of the differentiated SH-SY5Y cells at the 72 h time-point. Protein ID 8, TIMP2, was picked from the medium gel. The strip pI range was the same in all shown gels.