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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Integr Biol (Camb). 2010 Sep 1;2(10):517–527. doi: 10.1039/c0ib00054j

Figure 2.

Figure 2

Flow analysis and enrichment of reporter 3D μ-tissues. A) 3D sox17-dTomato ES cell μ-tissues cultured in differentiation media (-LIF) for 6d and analyzed by flow cytometry based on red peak height vs. total red fluorescence. Fluorescence-activated sorting was used to enrich for high-red (dTomato)-expressing ES μ-tissues, compared to low-red controls. B,C) Gated high and low-red ES μ-tissues collected into a multiwell plate. Fluorescence imaging for dTomato intensity (B) and quantitative RT-PCR analysis of sox17 expression (C) confirmed reporter fidelity. D) Gene expression analysis of post-sort μ-tissues revealed additional endoderm marker (foxa2 and gata4) enrichment, without enrichment for the neuroectoderm marker, pax6. Error bars represent s.d. of the mean (n=3). (Scale bar, 200 μm.)