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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nature. 2014 Mar 26;509(7498):101–104. doi: 10.1038/nature13134

Extended Data Figure 2. Protein expression of candidate reprogramming factors.

Extended Data Figure 2

a, Immunocytochemical detection of Bmi1, Hsf1 and Brg1 in zygotic interphase, M-phase and 2-cell interphase embryos. The expression pattern of these proteins was similar to that observed for mRNA expression (Extended Data Fig. 1d). 2-cell-I phase embryos were fixed 4 hours after cleavage. b, Western blot detection of Brg1 and Hsf1 in nuclear donor cells, intact and enucleated 2-cell embryos (n=40). c, Immunocytochemical detection of Brg1 and Hsf1 in nuclear donor FFs and ESCs. D, Immunocytochemical detection of Brg1 and Hsf1 in SCNT embryos. Z = zygote, M = mitosis, MII-metaphase II acolyte, I = interphone, FFs = fetal fibroblasts, ESCs = embryonic stem cells.