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Graphs summarizing tendon collagen content, cross-linking, and hydration. A hydroxyproline assay was used to determine the collagen content in the tendons, assuming 14% hydroxyproline per collagen molecule. Collagen cross-link analysis was performed using native fluorescence and high-performance liquid chromatography. Tendon hydration was determined by wet weight/dry weight measurements of lyophilized tissue. ACCS indicates amnion-derived cellular cytokine solution. *P > .05.
