Table 2.
HLA allelic associations with measures of rubella vaccine-specific immune response responses in the Rochester and San Diego study cohorts.
| HLA locus |
Allele | Neutralizing antibody titer (NT50/ml) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rochester cohort | San Diego cohort | |||||||||||
| Allele counts |
Median | 25th Percentile |
75th Percentile |
Allelic p- value |
Global p- value |
Allele counts |
Median | 25th Percentile |
75th Percentile |
Allelic p- value |
||
| Overall | 1398 | 57.4 | 35.4 | 95.7 | 2016 | 66.9 | 43.9 | 113.9 | ||||
| HLA-B | *27:05 | 58 | 48.9 | 34.2 | 88.4 | 0.067 | 0.056 | 55 | 54.8 | 39.4 | 93.1 | 0.047 |
| *50:01 | 12 | 111.3 | 31.4 | 277.3 | <0.001 | 12 | 49.7 | 45.0 | 64.5 | 0.099 | ||
| *57:01 | 35 | 48.7 | 34.2 | 69.9 | 0.022 | 48 | 59.1 | 43.4 | 93.8 | 0.697 | ||
| *58:01 | 7 | 109.8 | 95.1 | 136.8 | 0.025 | 34 | 100.4 | 64.4 | 133.7 | 0.289 | ||
| HLA-DPA1 | *01:04 | 7 | 93.4 | 66.1 | 178.7 | 0.053 | 0.010 | - | - | - | - | - |
| *02:01 | 213 | 54.0 | 34.6 | 87.3 | 0.015 | 367 | 66.8 | 43.8 | 113.3 | 0.561 | ||
| *01:09 | 13 | 4.6 | 1.7 | 18.8 | 0.092 | 171 | 65.4 | 44.8 | 111.0 | 0.960 | ||
| HLA-DPB1 | *03:01 | 140 | 45.5 | 32.1 | 77.4 | 0.004 | <0.001 | 136 | 54.5 | 36.6 | 92.0 | 0.267 |
| *04:01 | 567 | 61.6 | 38.2 | 104.6 | <0.001 | 608 | 70.8 | 44.6 | 113.3 | 0.084 | ||
| *06:01 | 30 | 34.1 | 27.0 | 64.2 | <0.001 | 26 | 57.2 | 39.9 | 112.9 | 0.396 | ||
| *15:01 | 9 | 121.4 | 67.5 | 178.7 | 0.005 | 8 | 78.0 | 43.0 | 163.5 | 0.677 | ||
| IFN-γ secretion (pg/ml) | ||||||||||||
| Rochester cohort | San Diego cohort | |||||||||||
| Allele counts | Median | 25th Percentile | 75th Percentile | Allelic p-value | Global p- value | Allele counts | Median | 25th Percentile | 75th Percentile | Allelic p-value | ||
| Overall | 1370 | 8.5 | 3.0 | 23.4 | 1896 | -1.4 | -6.4 | 3.1 | ||||
| HLA-DQB1 | *06:03 | 93 | 5.0 | 1.9 | 11.5 | 0.0003 | 0.045 | 90 | -0.7 | -5.9 | 4.9 | 0.576 |
| *51:01 | 64 | 5.5 | 1.4 | 17.0 | 0.039 | 94 | -0.5 | -6.6 | 4.5 | 0.037 | ||
Associations tested using linear models (linear regression for neutralizing antibody and linear mixed models for IFN-γ) using log-transformed antibody levels and inverse-normal transformations for IFN-γ. Analyses adjusted for age at blood draw quartile, quartiles for age at vaccination, time from most recent vaccination to enrollment, gender, race, and rubella vaccination assay run. Negative IFN-γ values indicate that the unstimulated secretion values were, on average, higher than the rubella virus-stimulated secretion values.