Figure 3.
Aqueous p-NPP hydrolysis activity of adsorbed (striped bars) and covalently-bound lipase on (trp)n-modified silica, during repeated reactions in the presence of 0.5% (v/v) Triton™ X-100. Prior to the first use, the covalent treatments were washed with 1% SDS in PBS (gray bars), or with PBS only (black bars). All samples were washed with PBS between runs.