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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Cell Mol Bioeng. 2010 Nov 12;3(4):361–368. doi: 10.1007/s12195-010-0145-8

FIGURE 2.

FIGURE 2

Aggregate formation is ICAM-1 specific and insensitive to initial cell ratios. RB143 and WERI-Rb27 retinoblastoma cell lines were used in the microtube aggregation assay. For both cell lines, RB cell concentration was held constant at 1 million/mL while neutrophil concentrations were varied from 2 million to 5 million/mL. Antibody blocking experiments were done using monoclonal anti-human ICAM-1 antibody. In the “E-selectin negative” experiments, the microtube surface was only incubated with protein G. In PMN-negative experiments, only RB cells were perfused in the functionalized E-selectin surface. ANOVA and student t-tests were used for statistical analyses. * p < 0.01; ** p < 0.005.