. 2014 Aug 7;10(8):e1003776. doi: 10.1371/journal.pcbi.1003776

Table 2. Energy costs (E) of HIV-1 NL4-3 Gag mutants predicted by computational models.

Mutant	Gag subunit	Ising E^a	Regularized Ising E^b	Regularized Potts E^c
186I	p24	78.74	9.98	11.24
269E	p24	43.43	11.46	12.18
186I269E	p24	Infinity	17.77	18.97
295E	p24	22.81	9.05	11.03
186I295E	p24	Infinity	15.36	17.79
181R	p24	44.62	13.55	12.12
310T	p24	6.26	5.87	7.20
181R310T	p24	Infinity	15.74	14.87
182S	p24	25.13	7.11	9.68
198V	p24	Infinity	12.32	-^d
182S198V	p24	Infinity	15.77	-^d
179G	p24	56.09	11.14	11.57
229K	p24	44.63	10.52	11.68
179G229K	p24	97.01	17.99	18.81
174G	p24	Infinity	15.47	11.71
243P	p24	66.65	11.1	11.08
174G243P	p24	Infinity	22.9	18.32
168I	p24	38.58	9.8	10.30
315G	p24	19.11	6.85	10.64
168I315G	p24	Infinity	14.78	16.39
331R	p24	11.77	7.37	9.17
186I331R	p24	Infinity	13.68	15.85
302R	p24	11.1	7.75	9.23
302R315G	p24	Infinity	12.4	15.30
315G331R	p24	Infinity	10.56	15.22
190I	p24	41.52	8.2	11.41
190I302R	p24	Infinity	12.28	16.12
219Q	p24	6.73	5.65	6.90
242N	p24	8.68	6.7	8.05
219Q242N	p24	10.8	8.04	10.07
146P	p24	7.22	5.62	6.26
147L	p24	3.42	4.25	6.54
146P147L	p24	6.58	5.77	4.74
326S	p24	4.59	4.78	5.69
310T326S	p24	10.53	7.72	8.81
173T	p24	5.92	5.81	7.02
173T286K	p24	4.89	6.56	7.75
173T286K147L	p24	4.12	5.93	6.78
12K	p17	3.74	1.91	4.38
12K54A	p17	4.84	3.19	5.63
86F	p17	8	4.53	6.00
92M	p17	8.74	6.01	9.43
86F92M	p17	Infinity	9.52	12.57

E is 2.98 for wild-type NL4-3 p24 and 3.43 for wild-type NL4-3 p17.

E is 3.67 for wild-type NL4-3 p24 and 1.64 for wild-type NL4-3 p17.

E is 4.43 for wild-type NL4-3 p24 and 2.81 for wild-type NL4-3 p17.

The 198V mutation was not observed within the MSA used to fit the Potts model, precluding the fitted model from assigning an energy to viral strains containing this point mutation.