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. 2014 Aug 7;10(8):e1004512. doi: 10.1371/journal.pgen.1004512

Figure 2. GCN-1 and ABCF-3 proteins are evolutionarily conserved functionally.

Figure 2

(A) Schematic representations of the GCN-1 and ABCF-3 proteins used in the yeast two-hybrid binding assays. Orange box, EF3-like domain. Green boxes, AAA domains. The growth of yeast on histidine-minus medium is summarized. +, wild-type growth. −, no or little growth. (B) Western blot analysis of immunocomplexes purified from wild-type animals with an anti-ABCF-3 antibody or control IgG. Both ABCF-3 and GCN-1 are present in the immunocomplex purified with an anti-ABCF-3 antibody but not with the control IgG. (C) Western blot analysis showing levels of ABCF-3 and GCN-1 proteins in fourth-larval stage animals of the indicated genotypes. (D) Western blot analysis showing the levels of eIF2α with phosphorylated serine 49 and total eIF2α of fourth-larval stage animals of the indicated genotypes. (E) Relative intensities of phosphorylated eIF2 to total eIF2 of fourth-larval stage animals of the indicated genotypes. Errors, standard deviations.