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. 2014 Aug 7;10(8):e1004543. doi: 10.1371/journal.pgen.1004543

Figure 2. Topo III overproduction, and recA and recQ deletions complement the growth and chromosome segregation defects in the gyrB(Ts) ΔtopA strain.

Figure 2

(a) Representative superimposed images of DIC and fluorescence pictures of DAPI-stained cells grown at the indicated temperatures as described in Materials and Methods. Size bars are 5 µm. Additional images are shown in Figure S1 and S2. (b) Spot tests were performed at the indicated temperatures. The LB plates were incubated for the indicated times. The strains used are all derivatives of RFM475 (ΔtopA gyrB(Ts)) except SB264 which is a derivative of RFM445 (gyrB(Ts)). They are: CT150 (RFM475 ΔrecQ), VU118 (RFM475/pPH1243), SB265 (RFM475 ΔrecA), VU454 (RFM475 ΔrecO) and SB264 (RFM445 ΔrecA). Cells carrying pPH1243 were grown in the presence of IPTG to overproduce topo III.