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. 2014 Aug 7;10(8):e1004543. doi: 10.1371/journal.pgen.1004543

Figure 3. Topo III overproduction and recQ deletion are epistatic to recA deletion in correcting the growth defect of the topA gyrB(Ts) strain.

Figure 3

Spot tests were performed at the indicated temperatures. The LB plates were incubated for the indicated times. The strains used are all derivatives of RFM475 (gyrB(Ts) ΔtopA). They are: a) CT150 (RFM475 ΔrecQ), SB265 (RFM475 ΔrecA) and VU492 (ΔrecQ ΔrecA); b) VU118 (RFM475/pPH1243), SB265 (RFM475 ΔrecA) and VU479 (SB265/pPH1243); c) VU118 (RFM475/pPH1243), CT150 (RFM475 ΔrecQ) and VU464 (CT150/pPH1243); d) RFM475, SB362 (RFM475 lexA3) and SB265 (RFM475 ΔrecA); e) SB362 (RFM475 lexA3), CT150 (RFM475 ΔrecQ) and VU501 (RFM475 ΔrecQ lexA3); f) RFM475 and SB262 (RFM475 recB::Tn10). Cells carrying pPH1243 were grown in the presence of IPTG to overproduce topo III.