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. 2014 Aug 7;10(8):e1004543. doi: 10.1371/journal.pgen.1004543

Figure 5. Effects of mutations affecting DNA replication on the growth and chromosome segregation defects in the gyrB(Ts) ΔtopA strain.

Figure 5

(a) Representative superimposed images of DIC and fluorescence pictures of DAPI-stained cells grown at the indicated temperatures as described in Materials and Methods. Size bars are 5 µm. Additional images are shown in Figure S1 and S2. (b) Spot tests were performed at the indicated temperatures. The strains used are derivatives of RFM475 (gyrB(Ts) ΔtopA). They are: VU155 (RFM475 oriC), VU188 (RFM475 dnaT) and VU176 (RFM475 holC2::aph).