Figure 1. Pie chart representing percent ratios of putative function-based groups of the tobacco genes activated during PTI.

156 putatively PTI-activated tobacco genes were found by suppression subtractive hybridization of samples taken 6 and 48 hours after inoculation of tobacco leaves with P. syringe hrcC- mutant bacteria. BLAST nucleotide similarity search (Altschul et al. 1990) was used to assign putative functions to the sequences. Based on the identified putative functions the genes were classified into 15 groups: cell structure (2), signaling (20), cellular protection (7), cell wall (11), phenylpropanoid (9), terpenoid (4), defense related (16), heat shock (9), protein metabolism (13), transport (12), metabolism and energy (21), photosynthesis (2), miscellaneous (7), unknown function (11), no significant similarity (32). The ratios and the corresponding percentages are demonstrated in the figure.