Table 1. cDNA microarray hybridization results of contigs identified from PTI-induced tobacco.
| Linear fold changes of PTI-related genes at 6 and 48 hpi measured by microarray | ||||
| Contig number | 6 h fold change | 48 h fold change | Genbank accession | Gene name |
| 1 | 5.51* | 3.86* | X74453 | N.tabacum OMT I-b mRNA |
| 2 | 2.99 | 8.02* | AB041519 | N. tabacum mRNA for P-rich protein, NtEIG-C29 |
| 3 | 0.98 | 1.56* | Y19032 | N. tabacum cell wall protein (TLRP tyrosine- and lysine-rich protein) |
| 4 | 1.34 | 3.99* | AF151215 | N. glauca cell-type guard cell glycine-rich protein |
| 5 | 11.5* | 7.51* | AB035125 | Nicotiana tabacum NtEIG-E17 mRNA for glycine-rich protein, complete cds |
| 6 | 7.18* | 4.98* | AB044153 | Nicotiana tabacum mRNA for peroxidase, complete |
| 7 | 4.77* | AB206920.1 | Capsicum annuum catf2 gene for acyl-transferase | |
| 8 | 5.9* | 3.84* | X78203 | H.muticus mRNA for glutathione S-transferase |
| 10 | 3.6* | AB041515 | Nicotiana tabacum NtEIG-E80 mRNA, complete cds | |
| 11 | 2.32* | 0.78* | M97360 | Nicotiana tabacum protein SAR8.2c |
| 12 | 2.93* | AF082893 | Solanum tuberosum methionine synthase (MS) | |
| 13 | 1.63* | 4.66* | AY422690 | N. attenuata tissue-type shoot beta-tubulin (TUB) |
| 14 | 2.23* | Z71395 | N.plumbaginifolia mRNA for calreticulin | |
| 15 | 2.96 | BT013651 | L. esculentum putative ABC transporter | |
| 17 | 3.49* | BT014186 | Lycopersicon esculentum clone 133363F | |
| 18 | 2.5* | AF127796 | Capsicum chinense acyl carrier protein (Acl1) | |
| 20 | 2.8* | 3.3* | AB061256 | Solanum tuberosum mRNA for NADP-dependent malic Enzyme |
| 21 | 2.03* | 4.66* | X70343 | N.sylvestris mRNA for extensin |
| 22 | 1.84* | 1.95 | AJ271872 | N. sylvestris Ext1.2B gene for extensin |
| 23 | 1.63* | 1.39 | BT013251 | L. esculentum putative ABC transporter |
| 24 | 2.03* | AB117525 | Nicotiana tabacum NtMKP1 mRNA for MAP kinase | |
| 26 | 6.47* | AJ309300 | Solanum tuberosum mRNA for putative membrane protein (poni2 gene). | |
| 27 | 3.06* | BT014512 | Lycopersicon esculentum clone | |
| 28 | 4.6* | L08830 | Lycopersicon esculentum BiP (binding protein)/grp78 (glucose-regulated prot.) | |
| 29 | 3.77* | L02124 | Nicotiana tabacum anionic peroxidase gene | |
| 31 | 7.19* | D86721 | Nicotiana tabacum DNA for glycine-rich | |
| 32 | 2.93* | AF001270 | Lycopersicon esculentum cytosolic NADP-malic enzyme (LeME2) mRNA | |
| 33 | 2.11* | 0.74* | NA | |
| 34 | 5.66* | AF307144 | Spinacia oleracea cytosolic 6-phosphogluconate dehydrogenase (pgdC) | |
| 35 | 5.95* | 6.52* | AJ538960 | Nicotiana tabacum cDNA-AFLP-fragment |
| 37 | 1.5* | NA | ||
| 38 | 3.39* | 2.94* | AY368274 | Nicotiana tabacum cyclophilin-like (CYP1) mRNA |
| 39 | 7.19* | AF150881 | Lycopersicon esculentum x L. peruvianum ferulate-5-hydroxylase (CYP84) | |
| 40 | 6.13* | AJ00321 | Solanum tuberosum mRNA for hypothetical protein | |
| 41 | 3.52* | AF321140 | Nicotiana tabacum S-adenosylmethionine synthase | |
| 44 | 2.53* | 3.12* | AB041516 | Nicotiana tabacum mRNA for P-rich protein EIG-I30 |
| 46 | 2.15* | X71441 | N.tabacum mRNA for cytochrome b5 | |
| 47 | 3.32* | 6.58* | X60058 | Nicotiana tabacum blp5 mRNA for luminal binding protein (BiP |
| 48 | 4.29* | 6.98* | NA | |
| 52 | 8.66* | U57350 | Nicotiana tabacum epoxide hydrolase mRNA, complete cds | |
| 53 | 0.7* | 0.49* | AY904339 | C. annuum calcium-dependent protein kinase 4 (CDPK4) |
| 54 | 1.25 | 2.43* | Z29529 | N. tabacum ethylene forming enzyme (EFE). |
| 56 | 1.54 | U91723 | N. tabacum 14-3-3 isoform b T14-3b | |
| 57 | 0.77 | DQ016993 | I. batatas putative L24 ribosomal protein | |
| 59 | 1.48* | 3.85* | AF290618 | N. glauca putative delta TIP (MIP2) |
| 60 | 1.87* | AK221707 | A. thaliana MAP3K-like protein kinase | |
| 61 | 2.9* | D26015 | N. tabacum CND41, chloroplast nucleoid DNA binding protein | |
| 62 | 2.04* | 1.9* | X58108 | L. esculentum mRNA for enolase |
| 63 | 2.99* | D26460 | Nicotiana glauca X Nicotiana langsdorffii mRNA for tumor-related protein | |
| 64 | 1.73 | D17467 | N. tabacumphenylalanine ammonia-lyase | |
| 67 | 1.33 | 1.40 | X66856 | N. tabacum MST1 mRNA |
| 68 | 2.29* | 1.73 | AY087786 | Arabidopsis thaliana clone 38412 mRNA, complete sequence |
| 73 | 4.05* | AF542544 | Nicotiana attenuata 5-epi-aristolochene synthase | |
| 76 | 1.82* | NA | ||
| 77 | 2.99* | AF082893 | Solanum tuberosum methionine synthase (MS) mRNA, complete cds | |
| 87 | 1.02 | BT012770 | L. esculentum clone 113748F | |
| 102 | 1.55* | X55974 | N.plumbaginifolia mRNA for superoxide dismutase | |
| 108 | 1.10 | Z29529 | N. tabacum ethylene forming enzyme (EFE) | |
| 111 | 2.24* | BT004527 | Arabidopsis thaliana At4g24190/T22A6_20 gene | |
| 116 | 3.14* | 3.26* | NA | |
| 122 | 3.36* | AF001270 | Lycopersicon esculentum cytosolic NADP-malic enzyme (LeME2) | |
| 124 | 3.07* | U91723 | Nicotiana tabacum 14-3-3 isoform b T14-3b Mrna | |
| 125 | 2.11* | Y11348 | M. sativa mRNA for annexin-like protein | |
| 126 | 4.1* | AB007907 | Glycine max. mRNA for 6-phosphogluconate dehydrogenase | |
| 127 | 1.61 | AF370549 | A. thaliana Putative ribosomal protein (F5A13.4) | |
| 128 | 1.30 | NA | ||
| 129 | 2.1* | NA | ||
| 130 | 2.17* | AF004233 | Nicotiana tabacum hydroxy-methyl-glutaryl-coenzyme A reductase (HMGRL) | |
| 132 | 1.72 | AF243180 | L. esculentum dicyanin | |
| 133 | 2.67* | L14594 | Catharanthus roseus heat shock protein 90 mRNA, | |
| 136 | 5.91* | X74452 | N.tabacum OMT I-a mRNA | |
| 137 | 2.8* | AB073628 | Nicotiana tabacum mRNA for receptor-like protein kinase, complete cds | |
| 156 | 0.72 | AJ421413 | N. tabacum mRNA for alpha-tubulin (tubA3 gene) | |
| 161 | 0.98 | 0.95 | AJ844617 | P. major mRNA for polyubiquitin (ubq3 gene). |
| 163 | 2.3* | 4.74* | AK121755 | Oryza sativa (japonica cultivar-group) cDNA |
| 165 | 1.34 | 3.71 | Z21796 | L. esculentum chorismate synthase 1 precursor |
| 169 | 1.18* | 0.83 | AB012636. | N. sylvestris Lhcb1*1 gene for light harvesting chlorophyll a/b-binding protein |
| 170 | 3.56* | 1.30 | AF001270. | Lycopersicon esculentum cytosolic NADP-malic enzyme (LeME2) |
| 171 | 1.01 | 1.15 | AJ223329 | N. tabacum TUQG3 gene, complete CDS. |
| 172 | 3.25* | U64823 | Nicotiana sylvestris amino acid permease (nsaap1) | |
Linear fold changes of the PTI-related genes in tobacco leaves treated with Pseudonas syringae pv. syringae hrcC- bacteria versus water controls were calculated based on 3 independent biological replicates. Base 2 logarithmic values of the ratios of medians of bacterium- and water-treated controls were used for student's T-test (p>0.05). Significant activation is designated by asterisks.