Table 1. Kinetic parameters for E. coli ADP-Glc PPase.

Assay Condition	Glc-1P		ATP-Mg		V max (U/mg)
	S 0.5 (mM)	n H	S 0.5 (mM)	n H
A. No effector	0.95±0.07	1.1	6.49±0.21	1.5	1.67±0.15
B. 25 mM Pyr	0.57±0.05	1.0	1.67±0.08	2.5	3.93±0.21
C. 50 mM Pyr	0.37±0.02	0.9	1.05±0.06	2.2	5.31±0.19
D. 25 mM Pyr +10 µM Fru-1,6-P2	0.23±0.02	1.3	0.99±0.07	3.6	31±2
E. 10 µM Fru-1,6-P2	0.77±0.04	1.0	5.02±0.25	2.1	6.21±0.19
F. 50 mM Pyr +150 µM Fru-1,6-P2	0.09±0.01	1.1	0.37±0.03	2.4	104±5
G. 150 µM Fru-1,6-P2	0.12±0.01	1.2	1.31±0.09	3.2	63±4
I. 2 mM Fru-1,6-P2	0.09±0.01	1.3	0.43±0.02	2.1	108±6
J. 50 mM Pyr +2 mM Fru-1,6-P2	0.11±0.01	1.0	0.19±0.02	1.4	110±5

Assays were carried out as described in Material and Methods. Values are average numbers from three independent experiments, using regression analysis.