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. 2014 Aug 7;8(8):e3084. doi: 10.1371/journal.pntd.0003084

Figure 1. Injection of BAF or MPA suppresses DENV infection in mosquito midguts.

Figure 1

A) DENV titers of bafilomycin (BAF; 5 µM and 25 µM)-injected mosquito midguts at 7 days post-blood meal (dpbm) were compared to those of the DMSO-injected control group. Each data point represents virus infection intensity (titer) from an individual midgut. Each mosquito was injected with 0.345 pmole (214.9 pg) or 1.725 pmole (1.1 ng) of BAF. B) DENV titers of mycophenolic acid (MPA; 50 µM and 250 µM)-injected mosquito midguts at 7 dpbm were compared to those of the DMSO-injected control group. Each mosquito was injected with 3.45 pmole (1.1 ng) or 17.25 pmole (5.5 ng) of MPA. C) DENV titers of mosquitoes injected with either 5 µM BAF, 250 µM MPA, or a cocktail of both compounds were compared to those of the control group. D–E) DENV titers of castanospermine (CAS, 250 mM and 1 mM)- or deoxynojirimycin (DNJ, 250 mM and 1 mM)-injected mosquito midguts at 7 dpbm were compared to those of the control group. F) DENV titers of BAF (5 µM) and MPA (250 µM)-injected mosquito salivary glands at 14 dpbm were reduced compared to those of the DMSO-injected control group. A–F) The red bar indicates a median value of titers. A–B, D–F) Mann-Whitney test. C) Kruskal-Wallis test. ***; p<0.001, **; p<0.01, *; p<0.05. Descriptive statistics for DENV infection assays are presented in supplementary table S2.