Figure 4. IFN-γ exposure increases immunoproteasome activities in HSG cells.

The cells were treated with IFN-γ (250 U/ml) for 24–96 hrs, lysed with triton X-100, and then subjected to proteasome activity assay using fluorogenic substrate, Suc-LLVY-AMC. At each experimental condition, three cell lysates samples obtained from three separate T25 flasks (n = 3) were analyzed. **, p<0.01.