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. 2014 Aug 7;9(8):e104135. doi: 10.1371/journal.pone.0104135

Figure 2. Acetylation sites of HATs on the Gata4 promoter.

Figure 2

The sequence of 1.0 kb at up-stream of the transcription start site of mouse Gata4 is indicated in Upper and band density is in the bottom. ChIP-PCR results run on agarose gel electrophoresis showed that five HATs (P300, CBP, PCAF, SRC1 and GCN5) proteins could bind to multiple sites on the promoter of Gata4 for possible acetylation of histone H3K9 on this site. All the five HATs (P300, CBP, PCAF, SRC1 and GCN5) were amplified with DNA fragments precipitated by anti-P300, anti-CBP, anti-PCAF, anti-SRC1 and anti-GCN5 antibodies. H3K9 and IgG were amplified with DNA fragment precipitated by anti-H3AcK9 antibody during the later with normal mouse IgG. Site 1: 43801992 bp∼43802137 bp, Site 2: 43802226 bp∼43802328 bp, Site 3: 43802338 bp∼43802475 bp, Site 4: 43802469 bp∼43802574 bp, Site 5: 43802817 bp∼43802972 bp.