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. 2014 Aug 7;9(8):e104135. doi: 10.1371/journal.pone.0104135

Figure 6. Anacardic acid inhibits histone H3K9 hyperacetylation under alcohol treatment.

Figure 6

Pregnant mice were exposed to various portions (0, 1.25, 2.5, 5 and 10 mg/kg) of anacardic acid to ascertain optimal dose. The result showed that the level of H3AcK9 on the Gata4 promoter after intraperitoneal injection with 5 mg/kg anacardic acid was the lowest compared with another dose. #: P<0.05 vs. blank group (n = 3) (A). ChIP-Q-PCR results further confirms that anacardic acid (5 mg/kg) could reduce significantly hyperacetylation of histone H3K9 induced by alcohol on the Gata4 promoter at E14.5. *: P<0.01 vs. control group (n = 3), **: P<0.01 vs. alcohol group (n = 3) (B). Western blot analysis showed that the anacardic acid (5 mg/kg) could inhibit significantly hyperacetylation of histone H3K9 in the myocardium. *: P<0.01 vs. control group (n = 6), **: P<0.01 vs. alcohol group (n = 6). (C): band density, (D): statistic analysis.