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. 2014 Aug 7;9(8):e104135. doi: 10.1371/journal.pone.0104135

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© 2014 Peng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The protein expression of α-MHC, cTnT and α-actin was analyzed using western blot that showed that alcohol could significantly increase α-MHC and cTnT expression at the level of protein in the mice heart exposed to alcohol during pregnancy. Meanwhile, anacardic acid could reverse over-expression induced by alcohol of α-MHC and cTnT protein. However, alcohol could not change the α-actin protein expression in the same samples. *: P<0.05 vs. control group (n = 6), **: P<0.05 vs. alcohol group (n = 6). (A): band density, (B): statistic analysis.