Figure 7. Effect of PGF2α on expression of Bax, Bcl-2 and Akt genes during PGF2α treatment.

(A) qPCR expression and analysis of Bax and Bcl-2 in the bovine CL. Mean (±SEM) fold expression changes before and after PGF_2α treatment is presented. Bars with different alphabets above them indicate statistical `significance (p<0.05). (B) Protein levels of Bax and Bcl-2 in the bovine CL. The CL tissue lysates were resolved on 10% SDS PAGE, transferred onto PVDF membrane and subjected to immunoblot analysis employing anti-Bax, anti-Bcl-2 and anti-β-actin antibodies. The immunoblot probe with β-actin antibody was used as a loading control. Densitometric values were determined and indicated as mean±SEM (n = 3 animals/time point), relative to intensity of β-actin for each time point post PGF_2α treatment. Bars with different alphabets indicate statistical significance (p<0.05). (C) Fold expression changes in mRNA and quantitative changes in protein as ratio of Bax/Bcl-2 levels in the luteal tissue during PGF_2α treatment. A representative immunoblot for each of the antibody probe is shown. Individual bars with different alphabets above them indicate statistical significance (p<0.05). Individual bars in solid box and open box represent mRNA expression and protein levels, respectively. (D) mRNA and protein levels of PI3k p85 in bovine CL. Mean (±SEM) fold expression changes in PI3k p85 mRNA examined by qPCR analysis. Quantitative changes in the ratio of pPI3k p85 and PI3k p85 protein levels were estimated. For the protein loading control, blots were probed with β-actin antibody. Densitometric values were determined and represented as mean±SEM (n =  3 animals/time point), relative to intensity of total PI3k p85 for each time point post PGF_2α treatment. Individual bars with different alphabets above them indicate statistical significance (p<0.05). Individual bars in solid box and open box represent mRNA expression and protein levels, respectively.