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. 2014 Aug 7;9(8):e104392. doi: 10.1371/journal.pone.0104392

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© 2014 Zhou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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After 14-day’s induction of hepatocyte differentiation, the treated cells were stained with immunofluorescence technique using individual human antibodies. The treated cells were positive while the untreated hUCMSCs negative of the following biomarkers: (A) hCK-18, (B) hCK-19, (C) hAFP, and (D) hALB. hCK-19 was negative in both cell types. These results confirmed that the treated cells had obtained a pattern in expressing a panel of genes which is consistent with the identity of hepatocyte lineage and could be classified as i-Hep. Nuclei were stained with Hoechst 33342. 200x.