Table 1.
Lower limit of quantification (LLOQ), upper limit of quantification (ULOQ), linear dynamic range (LDR) and standard curves for the three kits tested for interleukin-17A (IL-17) and interferon-gamma (IFNγ). Standard curves shown are based on extended dilution series with figures in brackets showing lower and upper limits suggested by each manufacturer where these differ. LLOQ, ULOQ and LDR were determined as described in Materials and methods.
| LLOQ (pg/mL) |
ULOQ (pg/mL) |
LDR (pg/mL) |
Standard
curve (pg/mL) |
||||
|---|---|---|---|---|---|---|---|
| IL-17 | VersaMAP | 5.9 | 4643.2 | 5.9 | 4643.2 | 0.2 (19.0) |
4616.0 |
| Bio-Plex | 1.3 | 23,036.0 | 1.3 | 5120.4 | 0.3 (1.3) |
21,505.0 | |
| MILLIPLEX | 3.4 | 8938.1 | 3.4 | 8938.1 | 0.2 (3.2) |
9000.0 (10,000.0) |
|
| IFNγ | VersaMAP | 0.3 | 5753.6 | 1.0 | 2397.6 | 0.3 (27.2) |
6620.0 |
| Bio-Plex | 8.1 | 30,659.5 | 26.5 | 7660.5 | 0.5 (1.9) |
30,646.0 | |
| MILLIPLEX | 2.8 | 9143.7 | 2.8 | 9143.7 | 0.2 (3.2) |
9000.0 (10,000.0) |
|