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. 2014 Aug 7;9(8):e104220. doi: 10.1371/journal.pone.0104220

Figure 8. Quantitative analysis of gene transcription in rats fed with different Diets.

Figure 8

(I) Bar plots showing the relative levels of (A–F) Lep-r, Hl, Cd36, Acc2, Cpt1a, and Pparα specific mRNA transcripts in hepatic samples from 4 rats/group. PCRs were performed in duplicate. Controls were set as 1 on the y-axis. (G) The diagram shows the fold change in the Irs-1 and Irs-2 gene expressions on the logarithmic y-axis vs. feeding categories. Actb (β-actin) and Ubc were used as an internal control. Lep-r: leptin receptor, Hl: hepatic lipase, Cd36: fatty acid transporter, Acc2: acetyl-CoA carboxylase 2, Cpt1a: carnitine palmitoyltransferase Iα, Irs: insulin receptor substrate. aP<0.05, aaP<0.01, aaaP<0.001 vs. Co, while b P<0.05 bbP<0.01 vs. LDC, cP<0.05 vs. L-Et, dP<0.05 vs. L-Fr, ***P<0.001 compared to the remaining groups.