Figure 2. Initial proliferation of M133 Tconv occurs in the DCLN.
(A) Experimental design. 1×105 CFSE labeled M133 Tconv (Treg-depleted CD4 T cells) were transferred to Thy1 congenic mice one day prior to rJ2.2 infection. (B) Representative plots showing proliferation of transferred cells and CXCR3 expression. (C) CFSE levels on transferred cells at several times p.i. MFI, mean fluorescence intensity. (D, E) Frequency (D) and numbers (E) of M133 Tconv at the indicated times after infection. The data are representative of four independent experiments with 3 mice per time point in each.