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. 2014 Aug 7;10(8):e1004278. doi: 10.1371/journal.ppat.1004278

Figure 3. Ixofin3D localization in the tick gut.

Figure 3

A. Purified Drosophila-expressed recombinant Ixofin3D-PF electrophoresed on SDS 12% polyacrylamide gel and Lane 1, Coomassie blue stained; Lane 2, Periodic Acid-Schiff stained; and Lane 3, rIxofin3D-PF immunoblotted and probed with polyclonal rabbit anti-Ixofin3D-PF serum. B. Confocal microscopy of PFA-fixed guts of 24 and 72h fed uninfected and B. burgdorferi-infected nymphs. Gut nuclei, B. burgdorferi and Ixofin3D stained with TO-PRO-3 (blue), anti B. burgdorferi (FITC-green) and anti-rIxofin3D-PF serum (TRITC-red) respectively. Magnification ×20. Guts stained with anti-Ovalbumin IgG (TRITC-red) served as antibody control. C. Mean pixel intensities of regions of interest in the TRITC channel (representing anti-rIxofin3D-PF serum binding to Ixofin3D) of the confocal images obtained in B. Each data point represents one region of interest. Error bars represent mean ± SEM and mean values significantly different in a one-way ANOVA with Tukey's multiple comparison test indicated by two asterisks (p<0.01) or indicated by three asterisks (p<0.0001).