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. 2014 Aug 7;10(8):e1004278. doi: 10.1371/journal.ppat.1004278

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© 2014 Narasimhan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Imunofluorescence microscopy of PFA-fixed in vitro grown B. burgdorferi to assess binding to rIxofin3D-PF. B. burgdorferi was detected with FITC-conjugated B. burgdorferi antisera (FITC-green), rIxofin3D-PF (Panel 1) was detected using rabbit anti-Ixofin3D-PF IgG (TRITC-red), and rIxophilin (Panel 2) was detected using mouse anti-rIxophilin IgG (TRITC-red). Magnification ×20. B. ELISA assessment of dose-dependent binding of rIxofin3D-PF to B. burgdorferi membrane protein extract-coated plates compared to rIxophilin, a tick protein that does not bind to Borrelia.