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. 2014 Aug 7;10(8):e1004278. doi: 10.1371/journal.ppat.1004278

Figure 6. RNA interference-mediated decrease in Ixofin3D results in decreased B. burgdorferi burden in the salivary glands and in murine skin.

Figure 6

Double-stranded ixofin3D (ds ixofin3D) or ds gfp was injected through the anal pore 3 h prior to B. burgdorferi-infected tick challenge (4–5 ticks/mouse). A. Engorgement weights of ticks fed to repletion. Each data point represents one tick; B. qRT-PCR assessment of ixofin3D expression; and C. Borrelia burden in tick guts and salivary glands. Each data point in B and C represents a pool of 3 guts or salivary glands; and D. qPCR assessment of Borrelia burden in murine skin at 7 and 14 days post-tick feeding. Each data point represents one mouse. Error bars represent mean ± SEM and mean values significantly different in a two-tailed non-parametric Mann-Whitney test (p<0.05) indicated by an asterisk. A representative of 3 experiments is shown.