Figure 7. VP4-induced permeability is comparable to that of virus.

Carboxyfluorescein-containing liposomes were mixed with VP4His at 5 µM (equivalent to approximately 5 µg/assay) or 1 µg HRV16 (equivalent to 50 ng VP4/assay) and membrane permeability detected by fluorescence measurements recorded every 30 seconds. Assays were conducted at 25°C (A) or 37°C (B). Only a minority proportion of recombinant protein is thought to take part in the reaction. Data is presented as % of total end-point release observed by lysis of liposomes by addition of detergent. Data shown is representative of multiple experiments (n>3).