. 2014 Aug 7;9(8):e102663. doi: 10.1371/journal.pone.0102663

Table 3. Identification of differentially expressed proteins.

No.		Protein Name	Gene	Accession No.	Protein MW (kDa)/PI					Protein Score C.I. %	GO	EC No.				Fold change
AC-RJM-up
22	SF2 CG6987-PA		SF2	gi\|66548276	28.4/9.88					100	F:nucleotide binding	-				↑∞
98	vasa intronic gene CG4170-PA, isoform A		Vig	gi\|66565092	46.9/8.85					99.69	P:RNA interference	-				3.22
104	glyceraldehyde-3-phosphate dehydrogenase 2 isoform 1		Gapdh	gi\|48142692	35.8/8.11					100	F:NAD/NADP binding; P:glucose metabolic process	EC:1.2.1.12				3.21
AC-RJM-down
15	Gamma-actin		Act5C	gi\|178045	25.8/5.65					100	P:adherens junction organization; F:protein kinase binding;	-				↓∞
21	ubiquitin conjugating enzyme E2		ben	gi\|156551253	17.3/5.71					100	P:response to anesthetic; F:ubiquitin-protein ligase activity;	EC:6.3.2.19				↓∞
77	abnormal CHEmotaxis family member (che-3) isoform 1		LOC411639	gi\|110757336	451.7/6.14					97.72	P:regulation of transcription, DNA-dependent; F:microtubule motor activity;	EC:3.6.1.3				↓∞
AM-RJC-up
54	pORF2		pORF2	gi\|16508047	149.5/9.7				99.97		P:RNA-dependent DNA replication; F:RNA-directed DNA polymerase activity;		-			↑∞
56	yellow-e2 CG17044-PA		yellow-e2	gi\|110776421	39.6/8.29				99.39		P:defense response to fungus/G+/G-; caste determination, influence by environmental factors;		-			↑∞
63	Hypothetical protein Bd0095		Bd0095	gi\|42521740	41.4/6.17				95		-		-			↑∞
64	mitochondrial malate dehydrogenase precursor isoform 1		LOC408950	gi\|66513092	35.8/9.33				100		P:tricarboxylic acid cycle; F:L-malate dehydrogenase activity;		EC:1.1.1.37			↑∞
85	Ornithine aminotransferase precursor CG8782-PA		Oat	gi\|66524972	47.3/8.5				99.99		P:metabolic process; F:ornithine-oxo-acid transaminase activity;		EC:2.6.1.13			↑∞
158	40S ribosomal protein S12		RpS12	gi\|66548338	15.3/5.48				100		P:translation; F:structural constituent of ribosome;		-			5.14
AM-RJC-down
67	Heat shock protein cognate 5 CG8542-PA		Hsc70-5	gi\|66501507	75.4/6.38			99.9			P:protein folding; F:2-alkenal reductase [NAD(P)] activity;		EC: 1.3			↓∞
68	thioredoxin peroxidase 1 CG1633-PA, isoform 1		Tpx-1	gi\|66548188	21.7/5.65			100			P:oxidation-reduction process; F:glutathione/thioredoxin peroxidase activity;		EC: 1.1			↓∞
69	Elongation factor 2 (EF-2) isoform 1		Ef2b	gi\|66508439	94.5/6.11			100			P: translational elongation; F:translation elongation factor activity;		-			↓∞
70	Fumarylacetoacetase (Fumarylacetoacetate- hydrolase)(Beta-diketonase)		Faa	gi\|66522657	41.6/6.09			100			P:aromatic amino acid family metabolic process; F:fumarylacetoacetase activity;		EC: 3.7			↓∞
71	Putative invasion protein		invA	gi\|16266003	7.4/9.64			97.68			P:nucleobase-containing compound metabolic process; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides		EC: 3.6			↓∞
86	T-complex Chaperonin 5 CG8439-PA, isoform A		Cct5	gi\|66522349	59.4/5.7			97.50					-			↓∞
100	hypothetical protein LOC726980		CG13321	gi\|110755727	14.4/5.52			99.99			F:transferase activity		-			3.21
101	40S ribosomal protein S12		Rps12	gi\|66548338	15.3/5.48			100			P:translation; F:structural constituent of ribosome;		-			3.19
102	60 kDa heat shock protein, mitochondrial-like		Hsp60	gi\|66547450	60.4/5.64			100			P:response to stress; F:solute:hydrogen antiporter activity;		-			6.64
103	Glyceraldehyde 3 phosphate dehydrogenase 1		Gapdh1	gi\|66517066	31.6/7.6			100			P:glucose metabolic process; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity;		EC: 1.2			3.27
136	hexamerin 110		Hex110	gi\|155369750	112.1/6.43			99.98			-		-			3.21
AC-RJC-CSBV-up
8	Vacuolar h		Vha55	gi\|66531434	55.1/5.41				99.57		P:ATP metabolic process; F:hydrogen-exporting ATPase activity, phosphorylative mechanism;		EC: 3.6			↑∞
9	HSP70		hsp70	gi\|92430370	70.8/5.42				99.97		P:response to stress; F:ATP binding;		-			↑∞
10	40S ribosomal protein S3a (C3 protein)		RpS3A	gi\|66547340	30.0/9.67				100		F:structural constituent of ribosome; P:translation		-			↑∞
72	MTA1-like CG2244-PB, isoform B		MTA1-like	gi\|110756803	94.4/9.68				95.86		F:zinc ion binding; P:regulation of transcription, DNA-dependent		-			↑∞
73	peroxidase-like isoform 2		pxd	gi\|110757934	87.6/7.11				99.053		P:response to oxidative stress; F:peroxidase activity;		EC: 1.1			↑∞
74	Argonaute 1 CG6671-PB, isoform B		AGO1	gi\|110777044	103.6/9.35				98.599		P:targeting of mRNA for destruction involved in RNA interference; F:miRNA binding;		-			↑∞
93	phosphatidylethanolamine-binding protein homolog F40A3.3-like isoform 1		LOC408516	gi\|66524882	20.6/8.84				99.793		-		-			3.55
133	60S ribosomal protein L9		RpL9	gi\|66565444	21507.7/9.88				99.962				-			3.17
132	10 kDa heat shock protein, mitochondrial-like		LOC552531	gi\|66547447	11.4/8.01				100		P:response to stress; F:ATP binding;		-			5.25
AC-RJC-CSBV-down
7	Hypothetical protein V12B01_09746		V12B01_09746	gi\|84388955		20.5/5.61				99.616	-	-				↓∞
89	tyrosyl-trna cytoplasmic		Aats-tyr	gi\|110762892		73.7/8.91				99.548	P:tyrosyl-tRNA aminoacylation; F:tyrosine-tRNA ligase activity;	EC: 6.1				3.64
91,128	fructose-bisphosphate aldolase		FBA	gi\|110748949		39.6/7.57				99.959	P:glycolysis; F:fructose-bisphosphate aldolase activity;	EC: 4.1				3.79 3.50
92	aldose reductase-like isoform 1		AKR-1	gi\|66525576		36.1/6.26				99.997	P:oxidation-reduction process; F:oxidoreductase activity	-				3.97
120	ribosomal protein s9		RpS9	gi\|48101950		22.5/10.74				97.835	P:translation; F:rRNA binding	-				4.47
122	peroxiredoxin 1		Jafrac1	gi\|66548188		21.7/5.65				95.152	P:hydrogen peroxide catabolic process; F: thioredoxin/glutathione peroxidase activity;	EC: 1.1				8.45
124	phosphoglycerate kinase		Pgk	gi\|110763826		44.9/8.15				100	P:phosphorylation; F:phosphoglycerate kinase activity;	EC: 2.7				9.74
125	60 kDa heat shock protein, mitochondrial-like		Hsp60	gi\|66547450		60.4/5.64				100	P:response to stress; F:solute:hydrogen antiporter activity;	-				3.45
126	t-box protein h15		mid	gi\|110757297		9.7/9.36				99.914	F:sequence-specific DNA binding transcription factor activity; P:regulation of transcription, DNA-dependent;	-				14.10
127	prohibitin protein wph		wph	gi\|48097857		29.9/6.54				100	Required for larval metabolism or for the progression of the larva into a pupa	-				3.72
129	pyruvate kinase		LOC552007	gi\|66548684		55.8/6.92				100	P:phosphorylation; F:potassium ion binding;	EC: 2.7				3.93
131	similar to CG31531-PA, isoform A isoform 1		LOC409705	gi\|66500352		113.1/6.62				98.889	P:imaginal disc-derived wing morphogenesis; F:zinc ion binding	-				3.09
AC-RJM-CSBV-up
13	eukaryotic translation initiation factor 5a		eIF-5A	gi\|110767655		17.6/5.19				97.017	F:translation initiation factor activity; P:translational initiation;	-				↑∞
14	Triosephosphate isomerase1		Tpi	gi\|148224276		26.9/7.77				99.999	P:gluconeogenesis; F:triose-phosphate isomerase activity	EC: 5.3				↑∞
87	neurochondrin homolog		CG2330RA	gi\|66509434		84.2/5.89				99.177	P:metabolic process; F:phosphoglycolate phosphatase activity	EC: 3.1				3.24
135	actin, indirect flight muscle-like		Act88F	gi\|110775533		37.3/5.37				99.948	P:phagocytosis, engulfment; F:ATP binding;	-				3.04
AC-RJM-CSBV-down
75	grip and coiled-coil domain-containing protein 1		CG10703	gi\|110750733		58.3/8.69				95.142.	P:cellular process	-				↓∞
76	60 kDa heat shock protein, mitochondrial precursor (Hsp60)		HSP60	gi\|66547450		60.4/5.64				100	P:response to stress; F:transporter activity;	-				↓∞
134	yellow-e2 CG17044-PA		CG15040	gi\|110776421		39.6/8.29				99.773	P:killing of cells of other organism;	-				3.36
AM-RJC-CSBV-up
31		lambda-crystallin homolog	Cryl1	gi\|66521282		34.4/6.3		100			F:3-hydroxyacyl-CoA dehydrogenase activity; P:oxidation-reduction process		EC:1.1.1.35			↑∞
32		GL19229	Dper\GL19229	gi\|194106483		17.3/6.05		99.505			-		-			↑∞
34		Catalase	Cat	gi\|25990773		57.9/8.39		100			P:hydrogen peroxide catabolic process; F:protein kinase activity;		EC:1.11.1.6			↑∞
82		metallo-beta-lactamase domain-containing protein 1-like	CG9117	gi\|110775625		16.1/5.86		95.029			F:hydrolase activity		-			↑∞
83		transposase	LOC725919	gi\|110766128		22.6/9.6		98.985			P:transposition, DNA-mediated; F:transposase activity;		-			↑∞
84		Translation elongation factor eEF-1 alpha chain	Eif	gi\|58585198		50.5/9.16		99.948			F:translation elongation factor activity; P:translational elongation;		-			↑∞
108		40S ribosomal protein S20	RpS20	gi\|110766823		13.7/9.95		100			P:translation; F:structural constituent of ribosome;		-			6.08
109		serine protease easter	Sp7	gi\|110757145		41.0/7.83		98.199			P:proteolysis; F:serine-type endopeptidase activity;		EC:3.4.21.0			5.08
110		atp synthase subunit mitochondrial	LOC551766	gi\|110762902		55.1/5.25		100			P:ATP synthesis coupled proton transport; F:ATP binding;		EC:3.6.3.6			3.80
111		CuZn superoxide dismutase	Sod	gi\|33089104		15.6/6.21		100			P:superoxide metabolic process; F:superoxide dismutase activity;		EC:1.15.1.1; EC:1.11.1.7			4.13
112		Nucleoside diphosphate kinase (NDK) (NDP kinase) (Abnormal wing disks protein) (Killer of prune protein)	Awd	gi\|66520497		17.6/6.75		100			P:nucleoside diphosphate phosphorylation; F:nucleoside diphosphate kinase activity;		EC:2.7.4.6			4.15
113		fructose-bisphosphate aldolase-like	FBA	gi\|110748949		39.6/7.57		100			P:glycolysis; F:fructose-bisphosphate aldolase activity		EC:4.1.2.13			3.49
114		ornithine aminotransferase, mitochondrial	Oat	gi\|66524972		47.3/8.5		100			P:metabolic process; F:ornithine-oxo-acid transaminase activity;		EC:2.6.1.13			4.46
33, 115,116,118		glyceraldehyde-3-phosphate dehydrogenase	LOC410122	gi\|66517066		31.6/7.6		100			F:NAD/NADP binding; P:glucose metabolic process;		EC:1.2.1.12			↑∞ 4.29 3.16 3.89
117		larval cuticle protein a3a-like	CPR31A	gi\|110764443		34.3/5.54		100			F:structural constituent of cuticle		-			3.25
119,137,138		glyceraldehyde-3-phosphate dehydrogenase 2 isoform 1	Gapdh	gi\|48142692		35.8/8.11		100			F:NAD/NADP binding; P:glucose metabolic process;		EC:1.2.1.12			8.54 3.08 3.14
AM-RJC-CSBV-down
24, 25, 26	60 kDa heat shock protein, mitochondrial precursor		Hsp60	gi\|66547450		60.4/5.64	100				P:response to stress; F:transporter activity		-			↓∞ ↓∞ ↓∞
27	60 kDa heat shock protein, mitochondrial		Hsp60	gi\|170045840		60.4/5.39	100				P: protein metabolic process; response to obiotic stimulus; F: nucleotide/protein binding; catalytic activity;		EC: 4.2			↓∞
30	GI16304		Hsp60	gi\|193906980		60.7/5.65	99.812				P: protein metabolic process; response to obiotic stimulus; F: nucleotide/protein binding; catalytic activity;		EC: 4.2			↓∞
78	Spectrin beta chain		Beta-Spec	gi\|110759783		268.6/5.37	97.139				F:phosphoprotein phosphatase activity;		EC: 3.1			↓∞
80	Stretchin-Mlck CG18255-PA, isoform A		Strn-Mlck	gi\|110757372		38.1/5.45	99.212				-		-			↓∞
81	yellow-h CG1629-PA		Yellow-h	gi\|110761428		33.7/5.71	100				-		-			↓∞
105	fatty acid binding protein		FABP	gi\|27531027		15.5/5.46	100				F:lipid binding; P:transport;		-			8.52
107	protein NPC2 homolog		NPC2	gi\|110756609		16.1/7.55	100				-		-			3.22
AM-RJM-CSBV-up
50	c6 transcription		SS1G_11931	gi\|156039589		50.2/8.99	97.085				F:sequence-specific DNA binding RNA polymerase II transcription factor activity; P:regulation of transcription from RNA polymerase II promoter;		-	↑∞
51,161	protein disulfide-isomerase		Pdi	gi\|110768510		24.3/4.74	100				F:electron carrier activity; P:cell redox homeostasis;		EC: 5.3; EC: 2.4		↑∞ 18.95
52	aldehyde dehydrogenase mitochondrial-like		Aldh	gi\|66526635		45.4/5.25	100				F:retinal dehydrogenase activity; P:oxidation-reduction process		EC: 1.2		↑∞
53	hydroxysteroid dehydrogenase-like protein 2-like		CG5590	gi\|66530010		45.2/8.69	100				P:oxidation-reduction process; F:glucose 1-dehydrogenase [NAD(P)] activity;		EC: 1.1		↑∞
153	heat shock protein cognate 3		Hsc70-3	gi\|229892214		72.8/5.29	100				F:metal ion binding; P:porphyrin-containing compound biosynthetic process;		EC: 4.2		4.15
154	phosphoglycerate kinase isoform 1		PGK	gi\|110763826		44.9/8.15	100				P:glycolysis; phosphorylation; F:phosphoglycerate kinase activity		EC: 2.7		6.13
155	a-kinase anchor protein 9		Cp309	gi\|110758909		81.4/8.39	98.602				P:microtubule cytoskeleton organization; F:protein binding;		-		7.52
156	heat shock protein cognate 4		Hsc70-4	gi\|229892210		71.0/5.43	100				F:ATP binding; P:response to stress;		-		4.99
157	glyceraldehyde-3-phosphate dehydrogenase 2 isoform 1		Gapdh	gi\|48142692		35.9/8.11	100				F:NAD/NADP binding; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; P:oxidation-reduction process;		EC: 1.2		3.68
161	26S proteasome non-ATPase regulatory subunit 13		Rpn9	gi\|66547365		43.6/6.07	95.252				-		-		18.95
163	myosin heavy muscle isoform 1		Mhc	gi\|110759191		224.7/5.78	99.86				P:ecdysone-mediated induction of salivary gland cell autophagic cell death; F:2-alkenal reductase [NAD(P)] activity;		EC: 1.3		3.14
AM-RJM-CSBV-down
35	30S ribosomal protein S3		RpS3	gi\|73661978		24.0/9.8	99.657				P:translation; F:RNA binding;		-		↓∞
37, 142,143	thioredoxin peroxidase 1 CG1633-PA		Jafrac1	gi\|66548188		21.8/5.65	100				P:response to stress; F:antioxidant activity;		EC: 1.1		↓∞, 3.36, 4.50
38	aldo-keto reductase		CG6084	gi\|66525576		36.1/6.26	100				P:metabolic process; F:catalytic activity		-		↓∞
36,39,42 141, 144145148	60 kDa heat shock protein, mitochondrial precursor		Hsp60	gi\|66547450		60.4/5.64	100				P:response to stress; F:transporter activity;		-		↓∞ ↓∞ ↓∞ 7.55 3.95 29.95 3.24
40	tyrosyl-trna cytoplasmic		Aats-tyr	gi\|110762892		73.7/8.91	100				P:translation; F:catalytic activity; RNA binding;		EC: 6.1		↓∞
43, 46	heat shock protein 60		Hsp60	gi\|156541962		60.5/5.5	100				P:response to stress; F:transporter activity;		-		↓∞ ↓∞
47	fructose-bisphosphate aldolase		FBA	gi\|110748949		39.6/7.57	100				P:carbohydrate metabolic process; F:catalytic activity		EC: 4.1		↓∞
49	heat shock cognate 70		Hsp70	gi\|66537940		71.7/5.43	100				P:response to stress; F:nucleotide binding;		-		↓∞
139	arylphorin subunit alpha		Lsp2	gi\|149939401		79.5/6.72	100				-		-		4.00
140	atp synthase subunit mitochondrial		Vha68	gi\|110762902		55.1/5.25	100				P:generation of precursor metabolites and energy; F:hydrolase activity;		EC: 3.6		3.07
147	similar to Pyruvate kinase CG7070-PB, isoform B		LOC552007	gi\|66548684		55.8/6.92	100								3.99
149	hexamerin 110		Hex110	gi\|155369750		112.1/6.43	100				-		-		4.27
150	protein NPC2 homolog		NPC2	gi\|110756609		16.1/7.55	100				-		-		6.33
151	grpe protein mitochondrial-like		Roe1	gi\|66525522		26.4/7.82	100				P:protein metabolic process; F:protein binding;		-		3.66
152	n-acetylneuraminate lyase-like		LOC725646	gi\|110755974		34.0/8.3	100				P:metabolic process; F:catalytic activity		-		4.19

Spot numbers and folds change correspond to the number of the protein spots in Figure 2. The theoretical molecular weight (Mr) and isoelectric point (pI) of the identified proteins were retrieved from the protein database of NCBInr limited to Apis mellifera. The Mascot score was searched against the A. mellifera database. Protein name is given when proteins were identified by MALDI-TOF/TOF-MS. The taxonomy is A. mellifera. Accession number is the unique number given to mark the entry of a protein in the NCBInr database. GO molecular function and biology process result in the Blast2GO analysis, P, Biological process; F, Molecular function.