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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: J Comp Neurol. 2011 Apr 1;519(5):972–988. doi: 10.1002/cne.22562

Figure 6.

Figure 6

Both SNAP25a and SNAP25b isoforms are expressed in mouse retina. Amplification of a 559-bp DNA product reflected the presence of total SNAP25 message (lane 2). Amplification of a 202-bp DNA product indicated the presence of SNAP25a (lane 3) and SNAP25b (lane 4) isoforms. The amplification of a 353-bp β-actin DNA product served as a control for the successful reverse transcription of mouse retina RNA into cDNA (lane 5). No spurious products are generated in the absence of the DNA template (lane 6). Lane 1, 100-bp DNA ladder; lane 2, total SNAP25; lane 3, SNAP25a, 202 bp; lane 4, SNAP25b; lane 5, β-actin control; lane 6, no template control.