TABLE 1.
List of Antibodies
| Antibody | Host | Antigen | Source | Dilution |
|---|---|---|---|---|
| Calbindin D-28k | Ms | Purified bovine kidney calbindin D-28k | Sigma (St. Louis, MO), C9848, clone CB-955 | 1:2,500–1:5,000 |
| Calbindin D-28k | Rb | Recombinant rat calbindin D-28k | Swant (Bellinzona, Switzerland), CB38 | 1:1,000 |
| Parvalbumin | Rb | Rat muscle parvalbumin | Swant, PV-28 | 1:2,000 |
| SNAP-25 | Gt | Recombinant human SNAP-25 | Millipore/Chemicon (Temecula, CA), AB5871P | 1:250–1:1,000 |
| SNAP-25 | Ms | SNAP-25 protein, human brain extracts, epitope ≈ C-terminus of SNAP-25 |
Covance/Sternberger Monoclonals (Emeryville, CA), SMI 81 | 1:5,000–1:500,000 |
| SNAP-25 | Ms | Human presynaptic protein precipitate | Sigma, S5187, clone SP12 | 1:5,000–1:10,000 |
| SNAP-25 | Ms | Recombinant full-length rat SNAP-25, epitope ≈ N-terminal amino acids 20–40 |
Synaptic Systems Göttingen, Germany), #111 011, cl. 71.1 | 1:20,000 |
| SNAP-25 | Ms | Recombinant full-length rat SNAP-25, epitope ≈ N-terminal amino acids 1–20 |
Synaptic Systems, #111 111, cl. 71.2 | 1:100–1:50,000 |
| SNAP-25 | Rb | N-terminal amino acids 9–29 human SNAP-25 | Sigma, S9684 | 1:7,000 |
| SNAP-25 | Rb | C-terminal amino acids 190–206 mouse SNAP-25 | BIOMOL/Affiniti Research Products (Plymouth Meeting, PA), SL3730 | 1:1,000–1:10,000 |
| SNAP-25 | Rb | C-terminal amino acids 190–206 mouse SNAP-25 | Millipore/Chemicon, AB1762 | 1:500 |
| SNAP-25 | Rb | C-terminal amino acids 195–206 mouse SNAP-25 | Assay Designs/StressGen Biotechnologies (Plymouth Meeting, PA), VAP-SV002 | 1:200–1:2,000 |
| VGAT-N2 | Rb | N-terminal amino acids 1–99 of VGAT, GST fusion protein | Dr. Robert Reimer, Stanford University School of Medicine (Palo Alto, CA) | 1:2,000 |