Figure 5.

brlA is not expressed and acetylation of histone H3K9/K14 at the brlA promoter is reduced in the ∆gcnE strain. (A) Both wild-type and ∆gcnE strains were grown vegetatively for 18 hr, and then conidiation was induced for 10 or 72 hr. RNA was isolated and gene expression was quantified by RT-qPCR. Data are shown normalized to the tubulin gene (benA) as an internal standard. (B) ChIP was carried out by immunoprecipitation of cross-linked DNA with an antibody recognizing acetylated histone H3K9ac and H3K14ac, followed by qPCR analysis of the promoter regions. brlA showed an increase in the immunoprecipitated DNA in both distal (brlAp1) and proximal (brlAp3) regions of the promoter in the wild type. In the ∆gcnE strain, acetylation levels were grossly reduced and conidiation-specific increases were not observed. Values were normalized to input DNA (before immunoprecipitation) and are shown as the mean with standard errors of the mean of at least three biologically independent experiments.