Table 1. Differentially regulated genes involved in development (sexual or asexual) and secondary metabolism in A. nidulans.
| WT vegetative vs. conidiation | ∆gcnE vegetative vs. conidiation | |||||
|---|---|---|---|---|---|---|
| Gene name | Gene code | qmediana | Log qmedian | qmedian | Log qmedian | Description of gene function |
| dewA | AN8006 | 160.8 | 5.1 | — | — | Hydrophobin, protein of the conidium wall responsible for hydrophobicity of conidium surface |
| yA | AN6635 | 28.2 | 3.3 | — | — | Conidial laccase (p-diphenol oxidase) involved in dark-green pigment production of conidium wall |
| pclA | AN0453 | 11.6 | 2.4 | 5.4 | 1.7 | G1/S cyclin: mutants produce abnormal conidiophores with extra layers of phialides. |
| ivoB | AN0231 | 11.1 | 2.4 | 3.7 | 1.3 | Conidiophore-specific phenol oxidase |
| wetA | AN1937 | 11.0 | 2.4 | — | — | Regulatory protein involved in conidial development |
| aflR | AN7820 | 10.0 | 2.3 | — | — | Transcriptional activator of the sterigmatocystin biosynthesis gene cluster |
| abaA | AN0422 | 8.9 | 2.2 | — | — | TEA/ATTS domain transcriptional activator involved in regulation of conidiation; required for phialide differentiation. |
| brlA | AN0973 | 5.9 | 1.8 | — | — | C2H2 zinc-finger transcription factor, master regulator of conidiophore development |
| rodA | AN8803 | 5.5 | 1.7 | — | — | Hydrophobin |
| medA | AN6230 | 5.3 | 1.7 | — | — | Protein involved in regulation of conidiophore development; required for normal temporal expression of brlA. |
| hogA | AN1017 | 4.2 | 1.4 | — | — | MAPK involved in osmotic stress response; required for sexual development and conidiation. |
| flbC | AN2421 | 3.7 | 1.3 | — | — | C2H2 zinc-finger transcription factor; involved in regulation of conidiophore development. |
| vosA | AN1959 | 3.6 | 1.3 | — | — | Nuclear protein involved in spore formation and trehalose accumulation |
| imeB | AN6243 | 0.4 | −0.9 | — | — | Serine/threonine protein kinase involved in light-mediated regulation of sexual development and sterigmatocystin production |
| nsdC | AN4263 | 0.3 | −1.2 | — | — | C2H2 zinc-finger transcription factor; required for sexual development. |
| laeA | AN0807 | 0.2 | −1.9 | — | — | Methyltransferase-domain protein: velvet complex component composed of VelB, VeA and LaeA; coordinates asexual development in response to light; regulates secondary metabolism; and is required for Hülle cell formation |
| pipA | AN2513 | 0.1 | −1.9 | — | — | Serine/threonine protein kinase involved in hyphal growth and asexual development |
| sskA | AN7697 | — | — | 0.2 | −1.6 | Response regulator, part of a two-component signal transducer involved in the HOG-signaling pathway that regulates osmotic stress response; null spores are heat labile and lose viability at 4° |
| rosA | AN5170 | — | — | 13.6 | 2.6 | Zn(II)2Cys6 transcription factor; negative regulator of sexual development |
—, not differentially regulated under those conditions in that strain.
a
qmedian values >1 (or positive log qmedian values) indicate that the gene is expressed during conidiation, while qmedian values <1 (or negative log qmedian values) indicate that the gene is expressed during vegetative growth. An absence of value indicates that gene is not differentially expressed in that strain.