Skip to main content
. 2014 Jun 10;197(4):1191–1200. doi: 10.1534/genetics.114.166926

Figure 7.

Figure 7

Overexpression of the formin BNI1 partially restores the shmoo formation defect of eIF5A mutants. (A) Overexpression of BNI1 does not restore the growth defect of eIF5A thermosensitive mutants at restrictive temperature. Growth of the indicated yeast strains containing a plasmid expressing BNI1 under the GAL promoter (pGAL-BNI1) was tested in Sgal −ura plates incubated at the indicated temperatures. (B) Immunoblots with antibodies against HA and hexokinase 2 protein (Hxk2) to show the expression of genomic Bni1 (BNI1) or the overexpression of Bni1 (pGAL-BNI1) at 25° or 37° for 4 hr. (C) Shmoo formation is partially restored in eIF5A mutants. The indicated strains containing a pGAL-BNI1 plasmid were grown at 25° in Sgal−ura media and then were maintained at 25° or transferred to 37° for 4 hr. Quantification of percentage of cells forming shmoos in samples treated with 10 μg/ml α-factor are indicated. Approximately 300 cells were manually counted for each sample from at least two independent experiments.