The function of the Set1 complex is essential for cells that have partially functional NIMA or CDK1 mitotic kinases. (A) Colony growth of strains of the indicated genotype at the semipermissive temperature of nimA7 (35°) after 96 hr and (B) at the semipermissive temperature of nimT23 (37.5°, resulting in partial NIMT and hence mitotic CDK1 function) after 96 hr. The inset shows magnified images of cells on the plate (Bar, 0.1 mm). Strains: WT = R153, nimA7 = MG71, nimT23 = MG99, ΔAn-swd1 = MG41, ΔAn-swd1 + nimA7 = MG65, ΔAn-swd1 + nimT23 = MG104; ΔAn-set1 = MG177, ΔAn-set1 + nimA7 = MG179, and ΔAn-set1 + nimT23 = MG181.