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Mutation of lysine 4 (K4) of histone H3 is lethal when NIMA or mitotic CDK1 function is reduced. Colony growth of the strains of the indicated genotype at the semipermissive temperature for nimA7 or nimT23 is shown after 96 hr. Strains: WT = R153, nimA7 = MG71, nimT23 = MG99, H3K4R = MG316, H3K4R + nimA7 = MG318, and H3K4R + nimT23 = MG267.
