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. 2014 May 15;197(4):1225–1236. doi: 10.1534/genetics.114.165647

Figure 3.

Figure 3

The G2–M transition in cells having partial mitotic CDK1 function requires An-swd1 and thus Set1 complex function. (A) Representative images of cells of the indicated genotypes carrying histone H1-chRFP and GFP-Tub grown at the semipermissive temperature for nimT23 (37.5°) and fixed for microscopic analysis. (B) Categorization of cells of different genotypes based on the number of nuclei. (C) Quantitation of average cell length of cells of each genotype as indicated. WT, n = 49; nimT23, n = 121; ΔAn-swd1, n = 110; and nimT23 + ΔAn-swd1, n = 114. (D) Calculation of the number of nuclei in 20 μm of cell length in WT cells compared to the single mutants and the double mutant. Strains: WT = HA365, nimT23 = HA375, ΔAn-swd1 = MG160, and nimT23 + ΔAn-swd1 = MG161.