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. 2014 May 15;197(4):1225–1236. doi: 10.1534/genetics.114.165647

Figure 4.

Figure 4

Cells lacking An-swd1 in addition to having partial mitotic CDK1 function exhibit a pronounced delay in initiating the first mitosis. (A) Live cell imaging of mitosis in a representative ΔAn-swd1 cell (strain MG276) resulting in two daughter nuclei. (B) Live cell imaging of mitosis in a representative cell lacking An-swd1 in addition to having partial CDK1 function (strain MG302) is successful in generating two daughter nuclei. Arrowhead indicates the spindle that continues to be visible in telophase. Images of representative live cell imaging of WT and nimT23 cells carrying H1-chRFP and GFP-Tub progressing through mitosis at the semipermissive temperature for nimT23 (37.5°) are shown in Figure S6. (C) Cell length at the time of entry into the first mitosis. WT (MG300), n = 13; nimT23 (MG304), n = 13; ΔAn-swd1 (MG276), n = 17; and nimT23 + ΔAn-swd1 (MG302), n = 15. (D) Time in mitosis as measured using GFP-Tub to monitor spindle formation and H1-chRFP chromosome condensation as markers for mitosis. WT, n = 12; nimT23, n = 13; ΔAn-swd1, n = 19; and nimT23 + ΔAn-swd1, n = 14. Bars, 5 μm.