Figure 1.

Pla2g2a polymorphisms identified in Apc^Min mice. (A) Chromatograph showing Pla2g2a promoter sequence for a short-lived (B6) and a long-lived (Polymorphic) Apc-mutant mouse. Polymorphisms designated by red arrows. (B) PCR-based assay for Pla2g2a polymorphisms identified in promoter sequence of long-lived (polymorphic) Apc-mutant mouse. (C) Screening different mouse strains for the Pla2g2a promoter polymorphisms using the PCR-based test. Strains AKR, DBA/2, DBA/1, C3H/He, BALB/c, FVB, and SJL carry the polymorphic promoter while strains C57, C58, A/J, P/J, and 129/Sv carry the B6 promoter. Het, heterozygous control sample from our mouse colony; −, PCR product without template; M, 100-bp DNA ladder. (D–G) Sequence results illustrate Pla2g2a polymorphisms found in different strains in the promoter area (D), first intron (E), exon 3 (F), and exon 4 (G). For exon 3 polymorphisms (E), sequence results illustrate the previously reported exon 3 nucleotide polymorphism marked by a black arrow; a newly identified R63W polymorphism in AKR and DBA/1 strains is marked by a blue arrow. Numbers represent nucleotide location, with +1 as the transcription start site.